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KMID : 0613820160260111245
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Journal of Life Science
2016 Volume.26 No. 11 p.1245 ~ p.1252
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Anti-inflammatory Effect of Myricetin from Rhododendron mucronulatum Turcz. Flowers in Lipopolysaccharide-stimulated Raw 264.7 Cells
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Choi Moo-Young
Hong Shin-Hyub
Cho Jun-Hyo
Park Hye-Jin
Jo Jae-Bum
Lee Jae-Eun
Kim Dong-Hee
Kim Byung-Oh
Cho Young-Je
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Abstract
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As a research of inflammation inhibitory activity using natural resource, the inflammation inhibitory activity by purified active compound from Rhododendron mucronulatum flower was experimented. Rhododendron mucronulatum flower components were purified and separated with Sephadex LH-20 and MCI gel CHP-20 column chromatography, Purified compound was confirmed as myricetin by 1H- NMR, 13C-NMR and Fast atom bombardment (FAB)-Mass spectrum to have inhibition activity on inflammatory factors secreted by Raw 264.7 cells in response to lipopolysaccharide stimulation. Myricetin inhibited nitric oxide (NO) expression in a concentration dependent manner, approximately 40% inhibition was observed at a concentration of 50 μM. The inhibition effect of myricetin on inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein expression was 20% and 80%, respectively, at a concentration of 25 μM. Myricetin also inhibited expression of the inflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and prostaglandin E2 (PGE2) in a concentration dependent manner; a concentration of 50 μM, 70%, 80%, 80% and 95% inhibition was observed, respectively. Therefore myricetin isolated from Rhododendron mucronulatum flowers is expected to have an anti-inflammatory effect in Raw 264.7 cell induced by lipopolysaccharides. The results can be expected myricetin from Rhododendron mucronulatum flower to use as functional resource for anti-inflammatory activity.
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KEYWORD
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Anti-inflammation, lipopolysaccharide, myricetin, purification, Rhododendron mucronulatum flowers
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